Office: room 123
Telephone: +30 210 772 4453
Fax: +30 210 772 3025
Address: National Technical University of Athens,
Iroon Polytechneiou 9, Zografou Campus,
Athens, 15780, Greece
DNA DAMAGE AND REPAIR LABORATORY
Dr. Alexandros Georgakilas received his bachelor’s degree in Physics with Honors at the University of Athens, Athens, Greece in 1992. He pursued post-graduate studies in molecular and cellular biology at the Institute of Biology, NCSR “Demokritos” in 1993. Following post graduate studies, Dr. Georgakilas received his Doctorate in Radiation Biology with Honors on the “Effects of a-Particles radiation on mammalian DNA”, Biology Department at the University of Athens, Greece in 1999. Subsequently, Dr. Georgakilas was a post-doctoral research associate in the Institute of Biology, NCSR “Demokritos” (1999-2000) and in the Department of Physics, Health and Medical Physics Group, Athens, Greece (2000-2001). Additionally, at the Brookhaven National Laboratory, Dr. Georgakilas completed post-doctoral research as an associate in the Biology Department from 2001-2003 under the supervision of Dr. Betsy Sutherland.
EXPERTISE and INTERESTS
Over recent years, Dr. Georgakilas’ has developed a reliable methodology for the study of clustered DNA lesions at the cellular or tissue level using human or bacterial DNA repair enzymes based on the pioneering work of Dr. B. Sutherland. There are limited data on the use of the DNA damage molecular assays for the detection of bistranded non-double strand break (DSB) oxidative clustered DNA lesions (OCDLs) in vitro or in vivo. Dr. Georgakilas work has provided a novel adaptation of neutral single cell gel electrophoresis (Comet assay) or pulsed field gel electrophoresis to measure these unique types of lesion and their repair. Thus far, Dr. Georgakilas’ research has contributed to the identification of novel DNA repair pathways for OCDLs, as well as uncovering different strategies that these mutagenic and repair resistant lesions are processed by the cell or human tissue. By this way, meaningful insights can be discovered for the biological and clinical application of DNA damage clusters. His interests are in the fields of biophysical analysis of DNA damage, biopolymers, oxidative stress and role of clustered DNA damage in carcinogenesis.
SELECTED PUBLICATIONS (OUT OF 52 TOTAL PEER-REVIEWED)
- Georgakilas, A. G., Bennett, P. V., Wilson III, D. M., and Sutherland, B. M. Processing of bistranded abasic DNA clusters in gamma-irradiated human hematopoietic cells. Nucleic Acids Res., 32: 5609-5620, 2004.
- Gollapalle, E., Wong, R., Adetolu, R., Tsao, D., Francisco, D., Sigounas, G., and Georgakilas, A. G. Detection of oxidative clustered DNA lesions in X-irradiated mouse skin tissues and human MCF-7 breast cancer cells. Radiat. Res., 167: 207-216, 2007.
- Peddi, P., Francisco, D. C., Cecil, A., Hair, J. M., Panayiotidis, M. I., and Georgakilas, A. G. Deficient processing of clustered DNA damage in human breast cancer cells MCF-7 with silenced DNA-PKcs expression. Cancer Lett., 269: 174-183, 2008.
- Redon, C., Dickey, J. S., Nakamura, A., Kareva, I., Naf, D., Nowsheen, S., Kryston, T. B., Bonner, W. M., Georgakilas, A. G., and Sedelnikova, O. A. Tumors induce complex DNA damage in distant proliferative tissues in vivo. Proc. Natl. Acad. Sci. USA, 107: 17992-17997, 2010.
- Peddi, P., Loftin, C. W., Dickey, J. S., Hair, J. M., Burns, K. J., Aziz, K., Francisco, D. C., Panayiotidis, M. I., Sedelnikova, O. A., Bonner, W. M., Winters, T. A., and Georgakilas, A. G. DNA-PKcs deficiency leads to persistence of oxidatively-induced clustered DNA lesions in human tumor cells. Free Rad. Biol. Med., 48: 1435-1443, 2010.
- Hair, J. M., Terzoudi, G. I., Hatzi, V. I., Lehockey, K. A., Srivastava, D., Wang, W., Pantelias, G. E., and Georgakilas, A. G. BRCA1 role in the mitigation of radiotoxicity and chromosomal instability through repair of clustered DNA lesions. Chem. Biol. Interact., 188 350-358, 2010.
- Aziz, K., Nowsheen, S., and Georgakilas, A. G. Nanotechnology in cancer therapy: targeting the inhibition of key DNA repair pathways. Curr. Mol. Med., 10: 626-639, 2010.
- Nowsheen, S., Wukovich, R. L., Aziz, K., Kalogerinis, P. T., Richardson, C. C., Panayiotidis, M. I., Bonner, W. M., Sedelnikova, O. A., and Georgakilas, A. G. Accumulation of oxidatively induced clustered DNA lesions in human tumor tissues. Mutat. Res., 674: 131-136, 2009.
- Georgakilas, A. G., Holt, S. M., Hair, J. M., and Loftin, C. W. Measurement of oxidatively-induced clustered DNA lesions using a novel adaptation of single cell gel electrophoresis (Comet Assay). Curr. Protoc. Cell Biol., 47 6.11.11-16.11.17, 2010.
- Kalogerinis, P., Poulos, J., Morfesis, A., Daniels, A., Georgakila, S., Daignualt, T., and Georgakilas, A. G. Duodenal carcinoma at the ligament of Treitz. A molecular and clinical perspective. BMC Gastroenterology, 10: 109, 2010.
- Georgakilas, A. G., Konsta, A. A., Haveles, K. S., and Sideris, E. G. Dielectric Study of the Double Helix to Single Coil Transition of the DNA Macromolecule. IEEE Trans. Dielectrics El., 5: 26-32, 1998.
- Kryston, T. B., Georgiev, A., and Georgakilas, A. G. Role of oxidative stress and DNA damage in human carcinogenesis. Mutat. Res., 711: 193-201, 2011.
- Georgakilas, A. G. Detection of clustered DNA lesions: Biological and clinical applications. World J. Biol. Chem., 2: 173-176, 2011.
- Sideridou, M., Zakopoulou, R., Evangelou, K., Liontos, M., Kotsinas, A., Rampakakis, E., Georgakilas, A. G., Moustakas, A., and Gorgoulis, V. G. Cdc6 acts as a “switch” at the E-cadherin locus linking transcriptional repression to activation of replication. J. Cell Biol., 195: 1123-1140, 2011.
- Aziz, K., Nowsheen, S., Pantelias, G., Iliakis, G., Gorgoulis, V., and Georgakilas, A. G. Targeting DNA damage and repair: Embracing the pharmacological era for successful cancer therapy. Pharmacol. Ther., 133: 334-350, 2011.
- Georgakilas, A. G. and Panayiotidis, M. I. Biomarkers of oxidative stress and cancer. From chemistry, biology to clinical applications and personalized therapy. Curr. Mol. Med., In press, 2012.
Special Issues and Books edited by Dr. Georgakilas
- Special issue on Mutation research
- Special issue on Current Molecular Medicine
- Book on Cancer Prevention (In Tech)
- Book on Cancer Biomakers (Science Publishers)
- Special Issue of Cancer Letters on 'OXIDATIVE STRESS-BASED CANCER BIOMARKERS'
- Special issue from Journal 'Cancers' on 'Role of Oxidatively-Induced DNA Damage in Carcinogenesis'
- Zacharenia Nikitaki (PhD student)
- Anastasios Maggelis (PhD student)
- Danae Laskaratou (Undergraduate student)
- Ifigeneia Mavragani (Undergraduate student)